Wednesday, October 21, 2009

which enzyme is not denatured over 60 degree centigrate?


Answers:
taq polymerase - used in the Polymerase Chain Reaction are from thermophylic microbes which were found in geisers and other hot places.
There are lots! For the bacteria and other life forms which have adapted to live at high temperatures, *all* of their enzymes have to be stable at those temperatures. Examples of such organisms would be the bacteria which live in the hot springs in Yellowstone and the life forms around the thermal vents in the ocean floor.The above mentioned polymerase from (I believe) T. thermophilus is an excellent example of how scientists have taken advantage of some of the heat stable enzymes found in nature. Just keep in mind that thermophilic organisms have thousands of other heat stable enzymes as well.
Taq Polymerase as one of the answerers said..
I just want to add that it's a DNA polymerase isolated from thermus aquaticus which naturally lives in hot springs.

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